CZ ID supports short- and long-read data from Illumina and Nanopore sequencing platforms. Below we summarize file requirements for both types of sequencing data.
- Short-read (Illumina) sequencing files:
-
- The mNGS Illumina pipeline accepts sequence data in FASTQ and FASTA format.
- If you are submitting short-read sequences in FASTA format, instead of FASTQ, files should be in a single-line format. That is, for each read, the second line, which specifies the DNA sequence, should be a single line without breaks.
- Files should contain no less than 70 reads and read lengths less than 50 base pairs or more than 500 base pairs are not supported.
- If uploading paired-end reads, the read length should be the same for both ends.
-
- Long-read (Nanopore) sequencing files:
-
- The mNGS Nanopore pipeline only accepts FASTQ sequencing files.
- You can only upload one file per sample. If you have multiple FASTQ files per sample, you need to concatenate data into a single file.
-