Does the metadata "nucleotide type" have an effect on the analysis pipeline?

Follow

Retchless, Adam (CDC/DDID/NCIRD/DVD)

• October 09, 2022 02:03

What effect, if any, is there from selecting "RNA" or "DNA" as the "nucleotide type" in the metadata. Many of my samples are from TNA with a reverse-transcriptase treatment, so the sequence data would include both DNA and RNA genomes.

reference:
https://chanzuckerberg.zendesk.com/hc/en-us/articles/360035296573-Upload-on-the-Web

0

• Official comment

  

  Elizabeth Fahsbender

  • January 05, 2023 22:23

  It does not affect the pipeline but the metrics that may be shown. For example, the mean insert size is only calculated for DNA libraries. However, this will not affect the taxa calls. 

  Comment actions Permalink

Please sign in to leave a comment.